Explain Podcast
🎙️The Explain Podcast is for everyone interested in DNA/RNA sequencing or technology.
Two colleagues from the West German Genome Center in Düsseldorf, Bioinformatician Daniel and Scientific Coordinator Iuliia, aim to EXPLAIN key next generation sequencing techniques and how these techniques contribute to our everyday life.
As Iuliia doesn’t have a sequencing background, she learns on the fly and keeps asking “simple” – even silly at times – questions…Daniel gives his best to clarify concepts in simple terms.
The Explain Podcast is recorded at the Multimediazentrum at Heinrich-Heine University of Düsseldorf.
Our podcast is an outreach activity within the national genomics facility ”West German Genome Center” funded by DFG No. 407493903 and MKW NRW.
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Enjoy and let us know what you think about our podcast!
✏️we’re happy to receive your feedback – write us podcast|dot|explain|at|gmail.com
YOU can contribute to making our Explain Podcast better – don’t miss a chance!
The Podcast Explain is for everyone interested in DNA/RNA sequencing or technology in general. As Iuliia doesn’t have a solid background in sequencing, she keeps asking “simple” – even silly at times – questions and Daniel gives his best to make us understand.
Spinnin' bases on wafers to do mostly natural SBS
Chapters:
– 00:00 Ultima ePCR with beads
– 07:10 Spinning the wafer, nucleotides, no blocking
– 14:30 Ultima ppmSeq + Element UltraQ
– 25:30 How Element UltraQ was born
– 30:30 snakemake, cromwell, nextflow
– 36:50 ncRNA
Ultimas homepage: https://www.ultimagenomics.com/
Nature paper with the chemistry: https://www.nature.com/articles/s41587-022-01452-6
Elements UltraQ chemistry:
Snakemake: https://snakemake.readthedocs.io/en/stable/
Nextflow: https://www.nextflow.io/docs/latest/index.html
Cromwell: https://cromwell.readthedocs.io/en/stable/
Small overview of not all ncRNA types: https://microbenotes.com/types-of-rna/
Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) – network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).
License Notice
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.
Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Sketches
License Notice:
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/