Welcome 🙂
🎙️The Explain Podcast is for everyone interested in DNA/RNA sequencing or technology.
Two colleagues from the West German Genome Center in Düsseldorf, Bioinformatician Daniel and Scientific Coordinator Iuliia, aim to EXPLAIN key next generation sequencing techniques and how these techniques contribute to our everyday life.
As Iuliia doesn’t have a sequencing background, she learns on the fly and keeps asking “simple” – even silly at times – questions…Daniel gives his best to clarify concepts in simple terms.
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf.
Our podcast is an outreach activity within the national genomics facility ”West German Genome Center” funded by DFG No. 407493903.
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Enjoy and let us know what you think about our podcast!
✏️ we’re happy to receive your feedback – write us podcast|dot|explain|at|gmail.com
YOU can contribute to making our Explain Podcast better – don’t miss a chance!
The Podcast Explain is for everyone interested in DNA/RNA sequencing or technology in general.
Bioinformatician Daniel and scientific coordinator Iuliia (both from West German Genome Center in Düsseldorf) aim to EXPLAIN key next generation sequencing techniques and how these techniques contribute to our everyday life. As Iuliia doesn’t have a solid background in sequencing, she keeps asking “simple” – even silly at times – questions and Daniel gives his best to make us understand.
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf.
Our podcast is an outreach activity within the national genomics facility ”West German Genome Center” funded by DFG (No. 407493903).
Chapters:
08:00 Gambling
15:00 Bubbles
30:00 Holes
57:30 One more thing
1:07:00 Finale
1:08:00 Finale of season 1!
Eric Chow explaining single cell sequencing: https://www.youtube.com/watch?v=k9VFNLLQP8c
Overview of things to consider: https://www.youtube.com/watch?v=VWUPKrnRvoQ
How to analyze single cell data: https://www.youtube.com/watch?v=5HBzgsz8qyk
A very good single cell analysis toolkit: https://satijalab.org/seurat/
Explainer for the rough workflow:
https://www.technologynetworks.com/genomics/articles/understanding-single-cell-sequencing-how-it-works-and-its-applications-357578
Quick explainer on single cell sequencing:
https://www.the-scientist.com/single-cell-sequencing-in-a-nutshell-71048
A method-overview paper: https://www.nature.com/articles/s12276-020-00499-2
Comparing BD Parse and 10xGenomics: https://pubmed.ncbi.nlm.nih.gov/33414681/
Pseudotime analysis: https://bioconductor.org/books/3.14/OSCA.advanced/trajectory-analysis.html
Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) – network of NGS core facilities in Germany.
Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ).
The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomics facility ”West German Genome Center” funded by the DFG (https://www.dfg.de/) (No. 407493903).
PS: I do not know how in the Parse approach the cells are treated to let the ligase and DNA enter each cell, but do not let RNA flow out of the cell at the same time. -DR
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