Explain Podcast
🎙️The Explain Podcast is for everyone interested in DNA/RNA sequencing or technology.
Two colleagues from the West German Genome Center in Düsseldorf, Bioinformatician Daniel and Scientific Coordinator Iuliia, aim to EXPLAIN key next generation sequencing techniques and how these techniques contribute to our everyday life.
As Iuliia doesn’t have a sequencing background, she learns on the fly and keeps asking “simple” – even silly at times – questions…Daniel gives his best to clarify concepts in simple terms.
The Explain Podcast is recorded at the Multimediazentrum at Heinrich-Heine University of Düsseldorf.
Our podcast is an outreach activity within the national genomics facility ”West German Genome Center” funded by DFG No. 407493903 and MKW NRW.
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Enjoy and let us know what you think about our podcast!
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YOU can contribute to making our Explain Podcast better – don’t miss a chance!
The Podcast Explain is for everyone interested in DNA/RNA sequencing or technology in general. As Iuliia doesn’t have a solid background in sequencing, she keeps asking “simple” – even silly at times – questions and Daniel gives his best to make us understand.
A long time coming, but here it is!
- Introduction: https://medlineplus.gov/genetics/understanding/howgeneswork/epigenome/
- Mechanisms: https://en.wikipedia.org/wiki/Epigenetics
- DNA packaging: https://www.nature.com/scitable/topicpage/dna-packaging-nucleosomes-and-chromatin-310/
- Chromatin and gene regulation: https://learn.genetics.utah.edu/content/epigenetics/control/
- Histone writers, erasers and readers: https://www.nature.com/articles/emm201711
- DNA methylation, DNMTs and SAM: https://www.nature.com/articles/npp2012112
- Modified bases (5mC, N6-mA): https://en.wikipedia.org/wiki/DNA_methylation
- Active DNA demethylation (TET/TDG/BER): https://www.tandfonline.com/doi/full/10.1080/17501911.2025.2586452
- Bisulfite sequencing (WGBS): https://www.illumina.com/techniques/sequencing/methylation-sequencing/bisulfite-sequencing.html
- WGBS alignment benchmarking: https://www.sciencedirect.com/science/article/pii/S2001037022003853
- Illumina 5-base methylome sequencing: https://www.illumina.com/techniques/sequencing/methylation-sequencing/methylome-genome.html
- MethylationEPIC v2.0 array: https://www.illumina.com/products/by-type/microarray-kits/infinium-methylation-epic.html
- Infinium 450K datasheet: https://www.illumina.com/content/dam/illumina-marketing/documents/products/datasheets/datasheet_humanmethylation450.pdf
- PacBio HiFi methylation detection: https://www.pacb.com/revio/
- SMRT direct methylation detection: https://www.pacb.com/publications/direct-detection-of-dna-methylation-during-single-molecule-real-time-sequencing/
- Fiber-seq and cancer research: https://www.pacb.com/blog/what-a-clearer-view-of-epigenetics-can-mean-for-cancer-research/
- PacBio epigenetics overview: https://www.pacb.com/products-and-services/applications/epigenetics/
- Sequencing 101: Epigenetics: https://www.pacb.com/blog/sequencing-101-epigenetics/
- Oxford Nanopore modified-base calling: https://nanoporetech.com/applications/investigations/epigenetics-and-methylation-analysis
- Nanopore sequencing accuracy: https://nanoporetech.com/platform/accuracy
- Nanopore chromatin accessibility (EPI2ME): https://epi2me.nanoporetech.com/chromatin-acc-hg002/
- Nanopore 5mC/5hmC detection: https://www.nature.com/articles/s42003-025-07681-0
- Ultima Genomics (bisulfite, EM-seq, TAPS+): https://www.ultimagenomics.com/focus-areas/methylation-profiling/
- Watchmaker TAPS+: https://www.watchmakergenomics.com/taps.html
- Element AVITI methylation sequencing: https://www.elementbiosciences.com/applications/dna-sequencing/methylation-sequencing
- MGI DNBSEQ bisulfite sequencing: https://link.springer.com/article/10.1186/s13148-023-01543-4
- NEBNext EM-seq v2: https://www.neb.com/en-us/products/e8015-nebnext-enzymatic-methyl-seq-v2-kit
- NEBNext enzymatic 5hmC-seq: https://www.neb.com/en-us/products/e3350nebnext-enzymatic-methyl-seq-5hmc-kit
- 5hmC detection overview: https://www.neb.com/en-us/products/epigenetics/methylation-analysis/5hmc-hydroxymethylation
- meCUT&RUN: https://www.epicypher.com/product/cutana-mecutrun-kit-for-dna-methylation-sequencing/
- Fiber-seq chromatin profiling: https://www.epicypher.com/fiber-seq-long-read-sequencing/
- Fiber-seq + PacBio HiFi: https://www.pacb.com/blog/how-pairing-epicyphers-fiber-seq-with-hifi-sequencing-delivers-an-all-in-one-multiomic-view/
- MeDIP: https://www.activemotif.com/catalog/736/medip
- LINE-1 global methylation assay: https://www.activemotif.com/catalog/1043/global-dna-methylation-assay-line-1
- RRBS: https://www.nature.com/articles/nprot.2010.190
- DNA methylation biomarkers in liquid biopsies: https://www.nature.com/articles/s41388-025-03624-5
Listen to our podcast and we’re happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/
Sketches
License Notice:
This podcast including all episodes, unless otherwise noted, is licensed under a Creative Commons Attribution 4.0 International License.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/